Composite

Part:BBa_K2443026:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)


EF-G optimized for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
    Illegal AgeI site found at 629
    Illegal AgeI site found at 1268
    Illegal AgeI site found at 1330
    Illegal AgeI site found at 1918
    Illegal AgeI site found at 1973
    Illegal AgeI site found at 2039
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for use in Escherichia coli. All regulatory elements are also optimal for use within Escherichia coli



Source

see basic parts

References